Human Carbonic Anhydrase B
نویسندگان
چکیده
منابع مشابه
Carbonic anhydrase in human platelets.
The carbonic anhydrase activity of human platelets was investigated by measuring the kinetics of CO2 hydration in supernatants of platelet lysates by using a pH stopped-flow apparatus. An average carbonic anhydrase concentration of 2.1 microM was determined for pellets of human platelets. Analysis of the kinetic properties of this carbonic anhydrase yielded a Km value of 1.0 mM, a catalytic-cen...
متن کاملIsolation of Human Carbonic Anhydrase B and C and Apocarbonic Anhydrase by Affinity Chromatography
Human carbonic anhydrase B and C have been purified in high yields by affinity chromatography, using two different affinity gels, incorporating the potent benzenesulfonamide inhibitor, but coupled to Sepharose through different ,spacer-arms~. Although both affinity gels bind carbonic anhydrase B and C quantitatively, they exhibit different binding capacities. Sepharose-glycyi-L-tyrosine-azobenz...
متن کاملDistribution of carbonic anhydrase among human photoreceptors.
The distribution of carbonic anhydrase (CA) among human photoreceptors has been a topic of dispute. In our experiments, by modifying an established enzyme histochemical technique, reproducible staining was observed. Of the cones in the peripheral retina, 91% were positive for CA. The CA-negative (CA-) cones were absent within approximately 8 arc min of the foveal center and their density peaked...
متن کاملPrimary structure of human carbonic anhydrase C.
The primary structure of human erythrocyte carbonic anhydrase C has been determined. The single polypeptide chain contains 259 amino acid residues devoid of disulfide bridges. The experimental approach has involved restriction of the action of trypsin to arginyl bonds by amidination of the lysyl side chains. The six tryptic fragments obtained have been separated and sequenced by manual techniqu...
متن کاملCarbonic anhydrase under pressure
Investigations of the rapid enzyme carbonic anhydrase have now been extended by crystallographic analysis at high CO2 pressures to examine the movements of water molecules in different steps of the catalysis. The rate of catalysis seems well explained by the assembled observations.
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1971
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)61810-1